Due to the concealed location of the pancreas, early pancreatic cancer is difficult to be found. Most patients are diagnosed at a late stage, and the 5 year survival rate is generally less than 5%. This cancer with fast growth and high metastasis rate is known as the “king of cancer”. However, the molecular mechanisms behind pancreatic cancer are poorly understood.
Transgenic mouse model is an ideal way to analyze the function of genes, which reveals the basic information for the development of pancreatic cancer. However, it takes a long time to build a transgenic mouse model. CRISPR/Cas9 delivery system based on transfection can rapidly and effectively multiple edit pancreatic genome in order to fully understand the mechanism of the development of pancreatic cancer.
CRISPR-Cas9 is originally an important defense mechanism in the evolution of bacteria in the long evolutionary history. Combination of clustered regularly interval short palindromic repeat CRISPR and enzyme cas9 can target and cut the intruder’s genetic material under the guidance of the guide RNA. CRISPR-Cas system is not only easy to operate, but also has a very strong scalability, fast penetrating into various fields of biological research.
Studies show that CRISPR/Cas9 multiple editing can achieve key pancreatic cancer research in mice, such as gene network analysis, synergistic lethal screening, chromosome engineering. The mouse model established by CRISPR/Cas9 can simulate chromosome deletion and inter chromosomal translocation, which can help people to understand the specific mutation of pancreatic cancer from many aspects. The researchers pointed out that the CRISPR/Cas9 delivery system based transfection can be a good summary of the nature of human tumors. It can be widely used in a variety of biological and preclinical studies.
For more than a decade, RNAi has been the trump card technology to study gene function, however, the emergence of emerging technologies is gradually collapsing the dominance of RNAi. Researchers can simply and intuitively find the most suitable one for their own technology according to their needs.